|
|
|
| Registros recuperados: 11 | |
|
|
| Frías-Quintana,Carlos Alfonso; Peña-Marín,Emyr Saul; Ramírez-Custodio,Carlos David; Martínez-García,Rafael; Jiménez-Martínez,Luis Daniel; Camarillo-Coop,Susana; Guerrero-Zárate,Rocío; Asencio-Alcudia,Gloria Gertrudys; Álvarez-González,Carlos Alfonso. |
| ABSTRACT In the Southeast of Mexico, there are many native cichlids with commercial interest such as redhead cichlid (Vieja melanurus) and twoband cichlid (V. bifasciata), which have a great local demand and excellent meat quality. However, it is necessary to implement their culture based on nutrition studies and digestive biochemistry. This study’s objective was to characterize these two cichlids’ digestive proteases (pH, temperature, and inhibitors) through biochemistry techniques. Results showed that V. melanurus and V. bifasciata have a digestive capacity analogous to other omnivore fishes, where the optimal pH values of stomach proteases (4 and 2, respectively) and intestinal proteases (6 and 12, respectively), the optimal temperature of acid (35°C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inhibitors; PH; Proteases; Stability; Temperature. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100210 |
| |
|
| |
|
|
| Santos,João Paulo Victorino; Araújo,Tatiane Ferreira; Ferreira,Célia Lúcia de Luces Fortes; Goulart,Simone Machado. |
| In this study, the potential of kefir grains, obtained from three different towns/cities in the Southeast Region of Brazil to inhibit the growth of the five pathogenic microorganisms was evaluated. The samples showed the growth inhibition from 42.80-69.15 for Staphylococcus aureus ATCC 6538, 30.73-59.89 for Escherichia coli ATCC 11229, 44.99-73.05 for Salmonela typhi ATCC 6539, 41.45-54.18 for Listeria monocytogenes ATCC 15313 and 70.38-86.80 for Bacillus cereus RIBO 1222-173-S4. These results indicated that the kefir grains evaluated had antagonistic activity toward the different pathogens tested. The ability to inhibit, although differently depending on the regional microbiota, indicated a potential for their use as a functional food. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kefir; Antagonism; Inhibitors; Functional foods. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500014 |
| |
|
| |
|
|
| Trottet, Aurore; Leboulanger, Christophe; Vidussi, Francesca; Pete, Romain; Bouvy, Marc; Fouilland, Eric. |
| The importance of heterotrophic bacteria relative to phytoplankton in the uptake of ammonium and nitrate was studied in Mediterranean coastal waters (Thau Lagoon) during autumn, when the Mediterranean Sea received the greatest allochthonous nutrient loads. Specific inhibitors and size-fractionation methods were used in combination with isotopic N-15 tracers. NO3 (-) and NH4 (+) uptake was dominated by phytoplankton (60 % on average) during the study period, which included a flood event. Despite lower biomass specific NH4 (+) and NO3 uptake rates, free-living heterotrophic bacteria contributed significantly (> 30 %) to total microbial NH4 (+) and NO3 (-) uptake rates in low chlorophyll waters. Under these conditions, heterotrophic bacteria may be... |
| Tipo: Text |
Palavras-chave: Bacteria; Phytoplankton; Inhibitors; Size fractionation; Nitrogen uptake; Dissolved inorganic nitrogen. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00314/42497/41907.pdf |
| |
|
| |
|
|
| Türkoğlu,E.A; Kuzu,M; Ayasan,T; Inci,H; Eratak,SV. |
| ABSTRACT Thioredoxin reductases (TrxRs) are selenocysteine-containing flavoenzymes that reduce Trxin NADPH-dependent manner. In the view of the direct vital role of TrxR in a wide range of biochemical and physiological processes, methods to inhibit this enzyme are clinically important. TrxR has recently emerged as a new candidate in anticancer drug investigations because of overexpression in tumorous cells. In this study, TrxR from chick liver was purified 94.6-fold with a yield of 4.86% and a specific activity of 0.19 EU/mg. KM and Vmax values of TrxR for DTNB were calculated as 0.9 mM and 0,03 EU/mL, respectively. Then, the effects of the flavonoids hesperidin, naringenin, chlorogenic acid, ferulic acid, naringin, 3,4-dihydoxybenzoic acid, and ellagic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flavonoids; Inhibitors; Natural products; Thioredoxin reductase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200345 |
| |
|
|
| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
| |
|
|
| Silva,Livia Cavaletti Corrêa da; Tamanini,Ronaldo; Pereira,Juliana Ramos; Rios,Edson Antônio; Ribeiro Junior,José Carlos; Beloti,Vanerli. |
| Milk fraud has been a recurring problem in Brazil; thus, it is important to know the effect of most frequently used preservatives and neutralizing substances as well as the detection capability of official tests. The objective of this study was to evaluate the analytical sensitivity of legislation-described tests and nonspecific microbial inhibition tests, and to investigate the effect of such substances on microbial growth inhibition and the persistence of detectable residues after 24/48h of refrigeration. Batches of raw milk, free from any contaminant, were divided into aliquots and mixed with different concentrations of formaldehyde, hydrogen peroxide, sodium hypochlorite, chlorine, chlorinated alkaline detergent, or sodium hydroxide. The analytical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fraud; Formaldehyde; Hydrogen peroxide; Chlorinated compounds; Inhibitors. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901613 |
| |
|
|
| Soares,A. M.; Oliveira,C. Z.; Santana,C. D.; Menaldo,D. L.; Silveira,L. B.; Teixeira,S. S.; Rueda,A. Q.; Marcussi,S.. |
| This work succinctly describes the professional and scientific life of Dr. José R. Giglio, one of the most outstanding Brazilian researchers in the field of Toxinology. During his long and successful career, he has made major contributions, especially in elucidating the function, structure, and mechanisms of action of animal venom proteins (from snakes, scorpions and spiders) as well as the characterization of antibodies and several inhibitors of venoms and toxins. We present here a brief history of Dr. Giglios personal and professional life, also reporting some of his numerous published scientific articles on venoms from snakes (Bothrops, Crotalus, and other genera), scorpions (Tityus sp), spiders (Phoneutria sp), their isolated toxins and natural... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxinology; Animal venoms; Toxins; Inhibitors; Homage; Giglio JR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400003 |
| |
|
|
| Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da. |
| Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025 |
| |
| Registros recuperados: 11 | |
|
|
|
